Fluid Chromatography Mass Spectrometry (LC-MS/MS) is an exceedingly delicate and explicit investigative procedure that can unequivocally decide the personalities and centralization of mixes inside your example. SCIEX mass spectrometers are at the cutting edge of quantitative and subjective examinations, and convey the speed and execution that guarantees your outcomes are as well as can be expected be. LC-MS is a strategy routinely utilized in test examination that joins fluid chromatography (LC) with mass spectrometry (MS). Amazingly touchy present day MS has helped LC-MS supplant a few immunoassays. LC-MS has improved the proficiency of medication disclosure because of its phenomenal affectability and particularity. The procedure can be joined with stable isotope weakening for exact and reproducible examines. Fluid chromatography–mass spectrometry (LC-MS) is an investigative science strategy that consolidates the physical partition capacities of fluid chromatography (or HPLC) with the mass examination abilities of mass spectrometry (MS). Coupled chromatography - MS frameworks are mainstream in synthetic examination in light of the fact that the individual abilities of every method are improved synergistically. While fluid chromatography isolates blends with various segments, mass spectrometry gives basic personality of the individual parts with high atomic explicitness and location affectability. This pair strategy can be utilized to break down biochemical, natural, and inorganic mixes normally found in complex examples of ecological and organic starting point. Along these lines, LC-MS might be applied in a wide scope of divisions including biotechnology, condition observing, food preparing, and pharmaceutical, agrochemical, and restorative ventures. Notwithstanding the fluid chromatography and mass spectrometry gadgets, a LC-MS framework contains an interface that productively moves the isolated segments from the LC segment into the MS particle source. The interface is essential on the grounds that the LC and MS gadgets are in a general sense contrary. While the versatile stage in a LC framework is a pressurized fluid, the MS analyzers usually work under high vacuum (around 10−6 torr/10−7 "Hg). Consequently, it is beyond the realm of imagination to legitimately siphon the eluate from the LC section into the MS source. By and large, the interface is a precisely straightforward piece of the LC-MS framework that moves the most extreme measure of analyte, evacuates a noteworthy bit of the versatile stage utilized in LC and jelly the concoction personality of the chromatography items (synthetically inactive). As a necessity, the interface ought not meddle with the ionizing proficiency and vacuum states of the MS framework. These days, most widely applied LC-MS interfaces depend on environmental weight ionization (API) systems like electrospray ionization (ESI), barometrical weight substance ionization (APCI), and air pressure photograph ionization (APPI).These interfaces opened up during the 1990s following a multi decade long innovative work process. Fluid chromatography-mass spectrometry (LC-MS) is currently a normal procedure with the improvement of electrospray ionization (ESI) giving a straightforward and vigorous interface. It tends to be applied to a wide scope of natural atoms and the utilization of couple MS and stable isotope inward measures permits profoundly delicate and precise tests to be created albeit some strategy advancement is required to limit particle concealment impacts. Quick examining speeds permit a high level of multiplexing and numerous mixes can be estimated in a solitary investigative run. With the improvement of progressively moderate and dependable instruments, LC-MS is beginning to assume a significant job in a few territories of clinical organic chemistry and contend with traditional fluid chromatography and different methods, for example, immunoassay.